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GBMSDG Meeting Archives

October 16, 2008

GBMSDG Annual Vendor Night & Dinner Meeting

Guest Speaker
Alfred L. Yergey
Laboratory of Cellular and Molecular Biophysics
National Institute of Child Health and Human Development, NIH

Spectral Variability - Why We Don't Get Better Answers

Hyatt Regency
575 Memorial Drive, Cambridge

Participating Vendors

Advion Biosciences, Agilent Technologies, Agilux Labs, Applied Biosystems, Apredica, Bruker Daltonics, Cambridge Isotope Laboratories, Cerno Bioscience, CovalX, Dionex, EquipNet, Eurofins, Gerstel, JEOL, LEAP Technologies, McKinley Scientific, Microliter Analytical Supplies, New Objective, Perkin Elmer, Protein Discovery, Quantum Analytics, Quest Pharma Services, Shimadzu, Tandem Labs, Thermo Fisher, Varian, Waters

Presentation Abstract

While the principal sources of variance in protein mass spectrometry are the inherent biological sample variability together with sample preparation and introduction, the role of variability in the mass spectra themselves is generally under appreciated. In this talk we present evidence for this fundamental variability as well as an approach that reduces it. The methodology that we have developed was primarily focused on using MALDI TOF measurements to assign the extensive posttranslational modifications of tubulin C-terminal peptides in reflector mode, but has been extended to MALDI TOF/TOF fragmentations and to the more challenging problems of MALDI linear spectra and ESI LC MS/MS studies.

About Alfred L. Yergey

Alfred L. Yergey, received a B.S. in Chemistry in 1963 from Muhlenberg College and a Ph.D in 1967 from The Pennsylvania State University under the direction of F.W. Lampe; he was a postdoctoral fellow at Rice University with J.L. Franklin from 1967-69. After 2 years at Esso Research and Engineering, he joined Scientific Research Instruments in Baltimore where he worked with a group led by Marvin Vestal in designing and producing the first commercial CI sources. He came to NIH in 1977 where he was active in the quantification of human metabolic kinetics of small endogenous molecules using Thermospray LC/MS including glucose, cortisol and acetylcholine. In addition, he was heavily involved in developing mass spectrometric, clinical and mathematical tools for the study of whole body calcium metabolism. He is currently a Section Chief in the NICHD and Adjunct Professor in the Faculty of Medicine of the University of Calgary. He has organized and taught short courses on Quantitative Mass Spectrometry and LC/MS for the American Society of Mass Spectrometry.

Dr. Yergeys current research interests lie in the areas of problems of MALDI fragmentation processes, protein characterization with emphasis on de novo sequencing and increasing mass spectral reliability. He and his group are also developing methods for determining cardiolipins in human serum by LC-MS.

Dr. Yergey is a member of the American Society for Mass Spectrometry, serving as Vice President for Arrangements from 1999-2001, the Canadian Mass Spectrometry Society and the Association of Biomolecular Resource Facilities (ABRF).