Career Opportunities
Post-Doc Research Position - Philips Electronics Nederland B.V., The Netherlands
Description
Development of novel validation tools for biomarker candidates based on 'Multiple Reaction Monitoring' by high-performance mass spectrometry
Philips Electronics Nederland B.V., The Netherlands Philips Research Europe (principal investigators: Dr. Ralf Hoffmann, Dr. Edwin Romijn) is working on protein biomarker discovery and protein biomarker validation in the area of oncology, and more specifically in the area of Prostate Cancer that will serve as content of the technology platforms capable of measuring biomarkers that are also being developed within Philips Research. Protein biomarker discovery and protein biomarker validation, especially when done in serum or plasma, is limited by mainly protein dynamic range, and overall sensitivity and often lack of high-quality antibodies against the target of interest.
The proposed post-doc research project will work on the development of novel approaches to deal with these aspects in relation to protein biomarker evaluation and validation, which overcomes part of the mentioned limitations. Multiple Reaction Monitoring (MRM) mass spectrometry coupled to multi-dimensional separation techniques and the use of stable isotopes for quantification purposes, in combination with bioinformatics tools to predict fragmentation behavior in a Triple Quadrupole Mass Spectrometer and retention behavior in multi-dimensional separations of biomarker candidates will be developed.
Biomarker candidates will come from several ongoing discovery activities making use of several technical approaches, ranging from COFRADIC, glycoproteomics, and 4-dimensional separation techniques. Scientific exchange at this level will primarily be with EMC and UOXF. Subsequent development of clinically applicable assays for selected candidates biomarkers for further validation in the Biomarker Validation subprogramme will be done in collaboration with U.Turku.
Requirements:
Recipient may not be citizen of the hosting lab.
Contact:
Info at www.pro-nest.org
